Biotic and Abiotic stress
Rasool Vahedi; Mohammad Mohsenzadeh Golfazani; Maryam Pasandideh Arjmand; Habibollah Samizadeh Lahiji
Abstract
Iron toxicity lead to increasing of free oxygen radicals, oxidative stresses and sever yield reduction of Rice. One of the plant responses for physiochemical and biochemical regulation to stresses is change of antioxidant enzyme contents. In this study the effect of five treatments of iron (Fe-EDTA) ...
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Iron toxicity lead to increasing of free oxygen radicals, oxidative stresses and sever yield reduction of Rice. One of the plant responses for physiochemical and biochemical regulation to stresses is change of antioxidant enzyme contents. In this study the effect of five treatments of iron (Fe-EDTA) (0, 100, 250, 400 and 500 mg/li-1) on relative expression of glutaredoxin (GRX), thioredoxin (TRX), peroxiredoxin (PRX) and catalase (CAT) genes of IR64 (susceptible) and Pokkali (tolerant) genotypes of Rice in Yoshida hydroponic media by Real time-PCR technique investigated. The results showed that the relative expression level of CAT gene in different levels of iron in Pokkali genotype was higher than IR64 genotype. The relative expression level of PRX in IR64 genotype in all of the levels was similar. The relative expression level of TRX in Pokkali genotype was not significant. The relative expression level of GRX in the most of stress levels in Pokkali genotype was higher than IR64 genotype. Low level iron result in increasing of relative expression level of CAT, GRX and TRX in IR64 genotype. But with increasing level of iron was not significant change in expression of genes. Also in the most of the iron level relative expression of genes in Pokkali was higher than IR64. Probably lose of change in gene expression levels in high level iron and low gene expression in IR64 is one of the reasons of its susceptibility to iron stress.
Biotic and Abiotic stress
Mohammad Mohsenzadeh Golfazani; Maryam Pasandideh arjmand; Mojtaba Kordrostami; Habibollah Samizadeh Lahiji; Hassan Hassani kumleh; Mohammad Hossein Rezadoost
Abstract
Increasing of reactive oxygen species (ROS) under iron toxicity is considered as one of the major constraints to rice production. In this study the alterations of SOD, GPX1 and MDHR expression level in two genotypes of rice, Pokkali (as tolerant) and IR64 (as sensitive) were monitored under different ...
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Increasing of reactive oxygen species (ROS) under iron toxicity is considered as one of the major constraints to rice production. In this study the alterations of SOD, GPX1 and MDHR expression level in two genotypes of rice, Pokkali (as tolerant) and IR64 (as sensitive) were monitored under different concentrations of iron levels [(0) (nonstress)], 100, 250, 400 and 500 mg/lit-1Fe-EDTA). The treatments were done when the plants were at 4-leaf stage and lasted for two weeks. Results showed that the expression levels of genes in Pokkali were higher than IR64. The expression level of SOD in IR64, increased at iron concentration increased, while it decreased at higher Fe-level. The expression level of GPX1 was increased in IR64, but decreased in Pokkali. The expression level of MDHR in IR64 was decreased at early stage of Fe-treatment, but then increased. Inversely, in Pokkali MDHR expression reduced constantly under Fe stress. Overall, the relative over expression of genes in Pokkali and presence of different expression levels of them between different concentrations of Fe in tolerant and sensitive genotypes indicate that the gene could remarkably effect on the tolerant level of pokkali by reducing ROS production under Fe-toxicity.
Biotic and Abiotic stress
Maryam Pasandide arjmand; Habibollah Samizadeh Lahiji; Mohammad Mohsenzadeh Golfazani
Volume 7, Issue 17 , July 2017, , Pages 31-42
Abstract
Drought is one of the most devastating environmental stresses that adversely affect plant metabolic processes. Many plant genes such as photorespiration ones are involve in response to drought stress. In the present study, the effects of drought stress on the expression level of two peroxisomal (Hydroxy ...
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Drought is one of the most devastating environmental stresses that adversely affect plant metabolic processes. Many plant genes such as photorespiration ones are involve in response to drought stress. In the present study, the effects of drought stress on the expression level of two peroxisomal (Hydroxy pyruvate reductase (HPR1) and Glycolate oxidase (GO)) and two mitochondrial (Serine hydroxy methyl transferase (SHMT) and Glycine decarboxylase (GDC)) genes were studied in susceptible (Hayola308) and tolerant (SLM046) genotypes of canola (Brassica napus) under stress (irrigation cut at flowering stage) and non-stress conditions. The result of real time-PCR showed that in Hyola308 genotype the expression level of GO gene at 48, 72 and 96 hours after stress was higher than SLM046 genotype. In Hyola308 genotype, the highest expression level of GDC gene observed at 48 hours of stress and then decreased. The highest relative expression level of SHMT gene in both Hyola308 and SLM046 genotypes detected at 24 hours after stress and then in SLM046 genotype, its level decreased at 48 hours after stress, while in Hyola308 genotype, its expression declined over the time of exposure to stress. SLM046 genotype showed highest amount of HPR1 expression level at 48 hours after stress. It seems that the expression of photorespiration genes in SLM046 genotype increased at the initial times of exposure to stress and with continue the stress, it showed more adaptation to stress and control the photorespiration unlike Hyola308.
